Top attune flow cytometer Secrets
Top attune flow cytometer Secrets
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Info-enriched characterization of cell populations. Morphological information and facts from photos can insert to your richness of flow cytometry details. For example, the figure shows an or else conventional apoptosis assay using Annexin V and PI, incorporating mobile imaging to characterize cells in Every single populace to expose morphologically distinctive options. These insights could not have already been obtained from multiplex staining by yourself.
As samples are obtained about the Attune CytPix Flow Cytometer, the high-pace brightfield digital camera captures and retailers photos of detected functions in a price as much as 6,000 images per second, based on the flow fee and graphic dimensions. For increased adaptability, Attune Cytometric Software allows you to alter the image seize frequency as essential and select a certain gate for graphic capture, if essential.
Blend acoustic concentrating technologies with large-parameter Assessment for flexible spectral unmixing and standard workflows.
Discover new insights into your experimental info by visualizing populations applying blended fluorescence and morphology details. Our software program interface wizards help downsampling, concatenation (merged information) and dimensionality equipment that are crafted to the software package for an intuitive, streamlined knowledge Investigation knowledge.
"An image is just not quite well worth 1000 dots, nonetheless it truly helps you to see photos. It is excellent to discover visuals that correlate with staining. For example, AnnexinV-optimistic cells which are morphologically various than wholesome cells, or CD14+ monocytes which can be greater and much more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it truly is illuminating to check out how many doublets slip by regular singlet gates, and good to verify the status of unexpected double-favourable situations in my analysis."
Pair your Attune flow cytometer by having an autosampler for improved workflow effectiveness, and add robotic integration for optimum throughput.
Consistent image high-quality even at superior throughput Acoustic concentrating and a significant-velocity camera Merge to graphic these T cells continuously at low or significant flow charges. Simply adjust emphasis and camera configurations to satisfy experimental needs.
Early indications of this contamination had initially been dismissed as debris. Backgating to find out new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also permits you to use morphological features to discover interesting subpopulations that may not be clear from flow cytometry facts by yourself.
Most likely most importantly, these situations contain cells of clearly various phenotypes which may lead to incorrect conclusions about double constructive gatherings (particularly in rare populations).
AI-pushed automated image analysis Using the Attune Cytometric Software interprets celebration functions into distinctive morphology parameters that can be coupled with conventional fluorescence and scatter parameters to reveal remarkably reproducible population adjustments and special subsets.
We Formerly shown the strength of imaging Automobile-T/Ramos cell interactions. Permit’s have a look at just the population of best fascination, the double optimistic gatherings, to learn more. We can easily now use extended morphological parameters (in this article, circularity and skewness of depth) to further more look at the features of those populations and refine gating on these activities, rising details robustness.
Leverage the strength of Highly developed technological know-how and innovation to speed up your discoveries. Have faith in in the Attune Flow Cytometer relatives to provide the reliability, speed, and precision that set your operate apart.
Adaptable payment prepare from Agilent Fiscal Remedies let you get the essential instrumentation you would like for speedy job, or to improve your lab.
Gating on these prolonged parameters means that you can speedily and correctly establish populations of interest and make sure gating approach with little if any handbook evaluate. The Attune computer software offers entry to condition-of-the-art equipment Discovering Examination equipment in an expertly created user interface, which makes it exceptionally easy to use.
Since the mobile line had Earlier been analyzed on the Attune CytPix Flow Cytometer, the scientists went again to the photographs and have been in the position to document the microbial an infection at least click here 5 days before.
Agilent Price Promise ensures you at least a decade of instrument use from the day you purchase. Usually, We'll credit you Using the residual worth of the program towards an upgraded product.
What appeared to be a homogeneous inhabitants is really additional diverse—an insight that should be thought of when interpreting results.